An evaluation of effectiveness of bio-glas in molecular sieving of polypeptides in guanidine hydrochloride |
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| Authors: | Aftab A Ansari Rose G Mage |
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| Institution: | Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014 USA |
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| Abstract: | The effectiveness of Bio-Glas 200 and 500 for separation and molecular weight estimation of polypeptide chains in 6 m guanidine hydrochloride was investigated. The distribution coefficients of 10 proteins after reduction and alkylation were determined. We found that the columns gave good calibration curves when pure proteins were passed individually. But the resolving power of Bio-Glas 200 and 500 in this solvent was very poor, apparently because of their high matrix volume and low inner volume. |
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