The synthesis of freezing-point-depressing protein of the winter flounder Pseudopleuronectusamericanus in Xenpuslaevis oocytes |
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Authors: | Choy-L Hew Cecil Yip |
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Institution: | 1. Department of Biochemistry, Memorial University of Newfoundland, St. John''s, Newfoundland, Canada.;2. Banting and Best Dept. of Medical Research, University of Toronto, Toronto, Canada. |
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Abstract: | The serum of the winter flounder contains a freezing-point-depressing protein of a molecular weight approximately 10,000 with 60% alanine in its composition. When injected into Xenopus o?cyte, a 6–10 S, poly A-rich RNA preparation isolated from the fish liver polysomes stimulated 3–4 fold the incorporation of 3H] alanine into 10% trichloroacetic acid-soluble, non-dialysable proteins. Analysis of the protein fractions showed a translation product similar in molecular weight and electrophoretic mobility to flounder freezing-point-depressing protein. These observations indicated that the 6–10 S RNA from the flounder contained mRNA for the synthesis of flounder's freezing-point-depressing protein. |
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Keywords: | FPDP freezing-point-depressing protein SDS sodium dodecyl sulfate |
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