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Des activites photosynthetiques sans les complexes pigmentaires CP1 et CP2
Authors:S Acker  A Picaud  J Duranton
Institution:1. Service de Biophysique, Département de Biologie, CEN Saclay, B. P. n° 2, 91190, Gif-sur-Yvette France;2. Service de Biochimie, Département de Biologie, CEN Saclay, B. P. n° 2, 91190, Gif-sur-Yvette France;3. Laboratoire de Photosynthèse, CNRS, 91190 Gif-sur-Yvette France
Abstract:Photosynthetic activity in the absence of the CP1 and CP2 pigmentary complexesVarious photochemical activities were tested on chloroplasts of Zea mays that received 4 s of light every 4 h during the culture period. Photosystem I and Photosystem II were functioning, as well as the photosynthetic electron transport. These chloroplasts exhibited upon sodium dodecyl sulphate gel electrophoresis neither Complex 1 (Mr 70 000) generally associated with Photosystem I nor Complex 2 Mr 25 000) generally associated with Photosystem II. Chlorophyll is indeed attached to polypeptides of molecular weight 21 000 and 29 000.These results lead us to question the functional role of chloroplast protein-pigment complexes observed by sodium dodecyl sulphate gel electrophoresis.
Keywords:DCPIP  2  6 dichlorophénol indophénol  DCMU  3  4-dichlorophényl diméthylurée  L1  plantules ayant reçu 4 s de lumière toutes les 4 h  V  plantules témoins ayant reçu un éclairage continu  CP1  CP2
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