Release of unsaturated vitamin B12 binding capacity from human granulocytes by the calcium ionophore A23187 |
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Authors: | James D Simon William E Houck Maurice M Albala |
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Institution: | 1. Division of Clinical Hematology, Rhode Island Hospital, Providence, R.I. 02902 USA;2. Brown University, Providence, R.I. 02902 USA |
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Abstract: | The ionophore A23187, in the presence of calcium ions, was capable of eliciting the release of granule-associated unsaturated vitamin B12 binding capacity from human granulocytes. The ionophore-induced extrusion of unsaturated vitamin B12 binding capacity was concentration, time and temperature-dependent. The release was blocked by 2-deoxyglucose and was unaccompanied by cytotoxicity (trypan-blue uptake and lactate dehydrogenase release). The unsaturated vitamin B12 binding capacity release was accompanied by the release of lysozyme, a specific granule marker enzyme. |
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