Isolation of two DNA polymerases from a mitochondrial membrane-DNA complex of mouse cell cultures |
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| Authors: | Klaus Radsak Gerhard Seidel |
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| Institution: | Hygiene-Institut der Philipps-Universität, Pilgrimstein 2, 355 Marburg/Lahn, Federal Republic of Germany |
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| Abstract: | A membrane-DNA complex isolated from the mitochondria of thymidine kinase deficient mouse cells could be shown to contain in addition to mitochondrial DNA two different DNA polymerases: (i) Mitochondrial DNA polymerase 1 exhibiting characteristics of the DNA polymerase described for HeLa cell mitochondria and (ii) mitochondrial DNA polymerase 2 showing properties comparable to those of the DNA polymerase isolated from mouse liver mitochondria. |
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