Identification of enzymatically produced peptide fragments by liquid chromatography and mass spectrometry |
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| Authors: | R Baranowski C Westenfelder B L Currie |
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| Institution: | 1. Section of Nephrology, Abraham Lincoln School of Medicine, University of Illinois at the Medical Center, Chicago, Illinois 60680 USA;1. Department of Medicinal Chemistry, College of Pharmacy, University of Illinois at the Medical Center, Chicago, Illinois 60680 USA |
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| Abstract: | The qualitative and quantitative determination of peptide fragments of angiotensin I generated by rat lung dipeptidyl carboxypeptidase (angiotensin converting enzyme, EC 3.4.15.1) is described. Enzymatically formed peptide fragments, after derivatization with fluorescamine, were separated and isolated by reverse-phase high-performance liquid chromatography. The recovered fluorescamine derivative of histidyl-leucine was then further identified by mass spectrometry. It is anticipated that this approach would be widely applicable to other enzyme systems. |
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