Effect of leukotriene B4, prostaglandin E2 and arachidonic acid on cytosolic-free calcium in human neutrophils |
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Authors: | P D Lew J M Dayer C B Wollheim T Pozzan |
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Institution: | 1. Infectious Diseases Unit, Immunology Unit and Institut de Biochimie Clinique, University of Geneva, Geneva, Switzerland;2. Istituto di Patologia Generale, Via Loredan 16, University of Padova, Italy |
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Abstract: | Changes in cytosolic free calcium Ca2+]i and release of beta-glucuronidase in response to leukotriene B4 (LTB4) were measured in intact neutrophils loaded with the fluorescent Ca2+ indicator, quin 2. LTB4 (10(-10) M or higher) caused a rapid rise in Ca2+]i due to influx from the extracellular medium and release from intracellular pools as well as enzyme release. PGE2 (3 microM) did not alter Ca2+]i whereas arachidonic acid (10 microM) raised Ca2+]i. Pretreatment of cells with the chemotactic peptide FMLP inhibited the subsequent rise of Ca2+]i induced by LTB4. Since chemotactic peptides activate the lipoxygenase pathway of arachidonic acid metabolism, it may be speculated that endogenous LTB4 generation is involved in neutrophil activation. |
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Keywords: | Prostaglandin Cytosolic free calcium Human neutrophil Quin 2 |
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