Production of Bacillus thuringiensis subsp. medellin by batch and fed-batch culture |
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| Authors: | F Vallejo A González A Posada A Restrepo S Orduz |
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| Institution: | (1) Biotechnology and Biological Control Unit, Corporación para Investigaciones Biológicas (CIB), A.A. 7378 Medellín, Colombia |
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| Abstract: | Bacillus thuringiensis subsp. medellin was grown until sporulation in a 1.1 l fermenter in batch and intermittent fed-batch culture. At optimum conditions 25 g dry cells l–1 and 9×108 spores ml–1 were produced. Toxicity of the final biomass showed a half lethal concentration on third instar Culex quinquefasciatus larvae of 295 ng ml–1. |
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| Keywords: | Bacillus thuringiensis subsp medellin fed-batch fermentation toxicity |
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