Establishment and characterization of a human malignant schwannoma cell line (HKMS) |
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| Authors: | I Ono I Ishiwata T Nakaguchi M Soma N Tokita C Ishiwata M Mukai S Nozawa H Ishikawa |
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| Affiliation: | Mutual Aid Association, Suifu Hospital, Japan. |
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| Abstract: | The malignant schwannoma cell line (HKMS) was established from the subcutaneous tumor of Axilla region of a 48-year-old Japanese woman. The HKMS line has the following biological properties. 1. The HKMS cells were spindle in shape and showed neoplastic and pleomorphic features. The monolayer sheet of HKMS cells showed the resemble cell-arrangement with that of the original tumor tissue. 2. The cells showed a stable growth and the serial passages were successively carried out 150 times within 3 years. Their population doubling time is about 40 hours. 3. The chromosome number varied widely, and the modal number was stable at the 78-80. The marker chromosomes were present. 4. The cells were transplanted into the subcutis of nude mice and produced the malignant schwannoma. |
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