Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome |
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| Authors: | Amelia A Langston Cathryn S Mellersh Cassandra L Neal Kunal Ray Gregory M Acland Mark Gibbs Gustavo D Aguirre REK Fournier Elaine A Ostrander |
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| Institution: | aDivision of Clinical Research, Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104;cDivision of Basic Sciences, Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington, 98104;bCenter for Canine Genetics and Reproduction, James A. Baker Institute for Animal Health, Cornell University, Ithaca, New York, 14853 |
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| Abstract: | We have constructed a collection of canine–rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome. |
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