Identification of Closely Related Hydrobaenus Species (Diptera: Chironomidae) Using the Second Internal Transcribed Spacer (ITS2) Region of Ribosomal DNA |
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Authors: | Hidetake Asari Shiro Kasuya Tadashi Kobayashi Shigeo Kondo Isao Nagano Zhiliang Wu |
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Institution: | 1. Gifu University , Japan;2. Institute for Environmental and Social Welfare Studies , Toyama, Japan;3. Aichi Medical University , Aichi, Nagakute, Japan;4. Gifu University School of Medicine , Aichi, Japan |
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Abstract: | Three species of Japanese Hydrobaenus Fries (H. Kondoi, H. biwaquartus and H. kisosecundus) can be separated by variation in the sequence of the second internal transcribed spacer (ITS2) regions of ribosomal DNA. Suggested synonymy of H. kondoi with H. biwaquartus is refuted, and previously suggested diagnostic morphology (virga length, antennal, leg and venarum ratios, shape of tergite IX and gonostylus length) distinguishes the taxa. No difference in larval morphology was found. H. kisosecundus is readily distinguished on adult and larval morphology from H. kondoi and H. biwaquartus, and is more distant by molecular similarity measures. ITS2 regions apparently provide useful information for distinguishing closely related species in Chironomidae. |
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