Production of herbicide-resistant transgenic maize plants using electroporation of seed-derived embryos |
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Authors: | Email author" target="_blank">Wagdy?A?SawahelEmail author |
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Institution: | (1) Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria |
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Abstract: | The improvement of commercial maize lines via biotechnological approaches is limited by the lack of a transformation system
that is tissue culture free. In this paper, the development of a genetic transformation system is presented using electroporation
for gene delivery and seed-derived embryo as the gene target. Plasmid DNA (pBARGUS), which contained the selectablebar gene for resistance to the herbicide Basta and the screenablegus gene, was delivered into enzymatically wounded mature maize embryos via electroporation. Transformed plants were identified
by their ability to grow on a selective medium containing 30 mg/L of phosphinothricin. Southern hybridization, plant resistance
to the application of Basta, GUS expression, and segregation analysis indicated that a functionalbar gene had integrated into the maize genome and was inherited in a mendelian fashion by the progeny. |
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Keywords: | DNA hybridisation electrotransformation herbicide-resistant gene mature embryo segregation analysis Zea mays |
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