Sequence-specific endonucleases in strains of Anabaena and Nostoc |
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Authors: | M. G. C. Duyvesteyn J. Korsuize A. de Waard A. Vonshak C. Peter Wolk |
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Affiliation: | (1) Department of Medical Biochemistry, Sylvius Laboratories, Wassenaarseweg 72, Leiden, The Netherlands;(2) MSU-DOE Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI, USA;(3) Present address: Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Sede Boquer Campus, 84990 Sede Boqer, Israël |
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Abstract: | The complements of restriction endonucleases of 12 strains of cyanobacteria were determined in cell-free extracts, and were compared with the complements of restriction activities assessed by measuring the relative efficiencies of plating of cyanophages on those cyanobacteria. The hosts which were susceptible to all of the phages contained endo R · AvaI and endo R · AvaII, and in several cases probably endo R · AvaIII, or isoschizomers of these enzymes. Three hosts which were lysed by only a subset (1 or 3) of the phages contained different restriction endonuclease. Anabaena sp. PCC 7120 showed apparent phenotypic restriction of phage An-22 grown in hosts with (isoschizomers of) AvaI, II and III, but no corresponding endonuclease has yet been detected in vitro. Nostoc sp. ATCC 29131 (PCC 6705) was found to contain a restriction enzyme, NspBII, with hitherot unknown specificity, C(A/C)GC(T/G)G. |
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Keywords: | Restriction endonucleases Cyanophages DNA recognition |
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