Catalytically inactive,purified RNase H1: A specific and sensitive probe for RNA–DNA hybrid imaging |
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| Authors: | Magdalena P. Crossley,Joshua R. Brickner,Chenlin Song,Su Mon Thin Zar,Su S. Maw,Fré dé ric Ché din,Miaw-Sheue Tsai,Karlene A. Cimprich |
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| Affiliation: | 1. Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA ; 2. Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA ; 3. Department of Molecular and Cellular Biology and Genome Center, University of California, Davis, Davis, CA |
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| Abstract: | R-loops are three-stranded nucleic acid structures with both physiological and pathological roles in cells. R-loop imaging generally relies on detection of the RNA–DNA hybrid component of these structures using the S9.6 antibody. We show that the use of this antibody for imaging can be problematic because it readily binds to double-stranded RNA (dsRNA) in vitro and in vivo, giving rise to nonspecific signal. In contrast, purified, catalytically inactive human RNase H1 tagged with GFP (GFP-dRNH1) is a more specific reagent for imaging RNA–DNA hybrids. GFP-dRNH1 binds strongly to RNA–DNA hybrids but not to dsRNA oligonucleotides in fixed human cells and is not susceptible to binding endogenous RNA. Furthermore, we demonstrate that purified GFP-dRNH1 can be applied to fixed cells to detect hybrids after their induction, thereby bypassing the need for cell line engineering. GFP-dRNH1 therefore promises to be a versatile tool for imaging and quantifying RNA–DNA hybrids under a wide range of conditions. |
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