Cellular Fatty Acid Analysis of Isolates of Bacillus thuringiensis Serovar kurstaki,Strain HD-1 |
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| Institution: | 1. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Fisheries College, Ocean University of China, Qingdao 266003, PR China;4. Institute of Biochemistry, Carleton University, Ottawa, ON, Canada, K1S 5B6;1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China;2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural, University, Tianjin 300384, PR China;3. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China |
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| Abstract: | Whole-cell cellular fatty acid (CFA) composition was utilized to determine if Bacillus thuringiensis serovar kurstaki (HD-1) larvicides produced by different manufacturers could be distinguished from each other and to determine whether these larvicides were distinguishable from isolates deposited in public collections. This study analyzed Biobit, Dipel, Foray, Thuricide, and the non HD-1 larvicides Delfin and Javelin, as well as the 1971 and 1980 Standards of HD-1. Isolates of HD-1 deposited in the collections of the American Type Culture Collection, Bacillus Genetic Stock Center, Pasteur Institute, and United States Department of Agriculture (USDA) were also analyzed. The data were grouped by hierarchical cluster analysis based on the unpaired-group method using arithmetic averages (UPGMA). Samples that linked at a Euclidean distance ≤2.0 units were considered to belong to the same fatty acid strain. Isolates of HD-1 from commercial products and deposits of HD-1 in the public collections and Standards were polytypic; 22 separate fatty acid strains were identified in the 1971 and 1980 Standards and 35 fatty acid strains were identified in the public collections. The type strain for Btk contained multiple fatty acid strains; three fatty acid strains were present in both the Bacillus Genetic Stock Center and the Pasteur Institute collections. In contrast, the type strain for serovar kurstaki in the USDA collection (HD-73) was monotypic and its fatty acid strain did not occur in other collections. We could distinguish between HD-1 and non-HD-1 larvicides using CFA composition. We conclude that CFA analysis may be used to identify commercial products. |
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