The effects ofPerkinsus marinusextracellular products and purified proteases on oyster defence parametersin vitro |
| |
Authors: | Perkinsus marinus |
| |
Institution: | School of Marine Science, Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, VA, 23062, U.S.A. |
| |
Abstract: | The effects of extracellular products (ECP) and purified proteases from the protozoan parasitePerkinsus marinuson three host defence parameters (haemocyte motility, lysozyme and haemagglutinin) of the eastern oyster,Crassostrea virginica, were investigated. ECP with high proteolytic activities, as well as purified proteases, significantly decreased the random migration of haemocytes through micro-porous filters in Boyden chambers. Stimulation of haemocyte migration byP. marinuscells orP. marinuscell lysate was also dramatically reduced by ECP and purified proteases. Incubation of oyster plasma with ECP and purified proteases caused a significant decrease in lysozyme activity and also appeared to reduce haemagglutinin titres. These data suggest thatP. marinusECP, as well as the proteolytic fraction of the ECP, can modulate some defence parameters of oystersin vitro. |
| |
Keywords: | Crassostrea virginica protozoa haemocyte motility lysozyme haemagglutinin |
本文献已被 ScienceDirect 等数据库收录! |
|