Immunochemical quantitation of isoenzymes. Alpha-amylase isoenzymes in barley malt

Two α-amylase isoenzymes were studied in germinating seeds from three varieties of barley by means of crossed immunoelectrophoresis, and amounts of corresponding isoenzymes could be compared. The method permits distinction between activator control and change in enzymatic protein quantity, de novo synthesis, and degradation. During germination of seeds variety Carlsberg II, two α-amylase isoenzymes were found to increase in amount to reach a maximum on Day 7 and then decrease.