Estimation of pH-dependence of calcium binding with calmodulin |
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Authors: | Danylovych Iu V Tuhaĭ V A |
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Institution: | Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv. kinet@biochem.kiev.ua |
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Abstract: | A methodical approach to estimating calmodulin Ca(2+)-binding properties based on its interaction with highly porous watman and consequent 45Ca2+ binding was proposed. At changing pH from 6.5 until 7.5 the affinity of Ca2+ to calmodulin increases in 4.3-fold. The article displays a model of mechanism for Ca(2+)-binding with calmodulin where the dissociation of H+ from Ca(2+)-binding sites is a limited stage of the process. |
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