Expression of the mouse testicular histone gene H1t during spermatogenesis |
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Authors: | B Drabent C Bode B Bramlage D Doenecke |
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Institution: | 1. Zentrum Biochemie, Abteilung Molekularbiologie, Universit?t G?ttingen, Humboldtallee 23, D-37073, Gottingen, Germany
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Abstract: | The testicular H1 histone variant, H1t, is synthesized during spermatogenesis in mammalian male germ cells. In situ hybridization
and immunohistochemical techniques were used to assign the expression of either the H1t mRNA or the H1t protein to specific
cell stages of spermatogenesis. Our results show the presence of the H1t mRNA only in the late and mid-pachytene stages, whereas
the protein occurs first in pachytene spermatocytes, and persists until later stages from round up to elongated spermatids.
Accepted: 1 March 1996 |
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