Laboratory and field methods for measurement of hyphal uptake of nutrients in soil |
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Authors: | P Schweiger I Jakobsen |
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Institution: | 1. Department of Plant Biology and Biogeochemistry, Ris? National Laboratory, DK-4000, Roskilde, Denmark 2. Department of Microbial Ecology, Lund University, Ecology Building, S-223 62, Lund, Sweden
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Abstract: | Experimental systems for measuring nutrient transport by arbuscular mycorrhizal (AM) fungi in soil are described. The systems
generally include two soil compartments that are separated by fine nylon mesh. Both roots and root-external hyphae grow in
one compartment, but only hyphae are fine enough to grow through the mesh into the other compartment. Application of tracer
isotopes to the soil of this hyphal compartment can be used to measure nutrient uptake by plants via AM fungal hyphae. Use
of compartmented systems is discussed with particular reference to phosphorus, which is the mineral nutrient transported in
the largest quantity by AM fungi. Laboratory and field applications of the compartmentation methodology are presented with
emphasis on the functioning of native AM fungal communities. Advantages and limitations of the method are considered and future
important research directions are discussed in this context.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Arbuscular mycorrhizal fungi field studies hyphal phosphorus transport |
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