Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis |
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Authors: | Ferrer Elizabeth González Luis Miguel Foster-Cuevas Mildred Cortéz Maria Milagros Dávila Iris Rodríguez Mercedes Sciutto Edda Harrison Leslie J S Parkhouse R Michael E Gárate Teresa |
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Affiliation: | Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain. |
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Abstract: | A cDNA encoding for a predicted small heat shock protein (sHSP), Tsol-sfISP35.6, has been isolated by antibody screening of a Taenia solium c-DNA library. The clone was a full-length sequence (1172 bp) with an open reading frame of 945 bp and encoded for a 314 amino acid protein with deduced molecular mass of 35.6 kDa, isoelectric point of 5.6 arid the characteristic HSP20/alpha-crystallin domain duplicated. It was highly conserved, with a high sequence similarity with other platyhelminth sHSPs. Western blot analysis, using serum from neurocysticercosis patients (NCC), indicated that the purified Tsol-sHSP35.6 expression product was immunogenic, while in indirect ELISA, using the purified Tsol-sHSP35.6 expression product as antigen and serum samples from pigs and humans, 80% of T. solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed. |
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Keywords: | Taenia solium Metacestode Cestode Small heat shock protein Recombinant antigen Diagnosis Pathogenesis sHSP, Small heat shock protein NCC, Neurocysticercosis CNS, Central nervous system CSF, Cerebrospinal fluid E/S, Excretory/secretory antigen ELISA, Enzyme-linked immunosorbent assay |
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