Prostaglandin levels in BL6 melanoma cells cultured in vitro: the effect of vitamin E succinate supplementation |
| |
Authors: | P Ottino JR Duncan |
| |
Institution: | Department of Biochemistry and Microbiology, Rhodes University, PO Box 94, J Grahamstown, 6140, South Africa |
| |
Abstract: | Malignant murine melanoma (BL6-F10) cells convert arachidonic acid primarily to PGD2, PGF2α, PGE2, PGI2 in descending order of magnitude. Supplementation with 1–10 μg/ml vitamin E succinate resulted in a significant (P ≤ 0.05) decrease in PGD2 levels at vitamin concentrations of 3,5,7 and 10 μg/ml respectively, while PGF2α levels were significantly decreased at 1,3,5 (P ≤ 0.05), 7 and 10 μg/ml (P ≤ 0.01) vitamin E succinate. BL6-F10 cells supplemented with 7 and 10 μg/ml vitamin E succinate showed a marked increase in PGE2 levels with a significant increase occurring at 10 μg/ml (P ≤ 0.025). PGI2 levels followed a similar trend to PGE2 with a significant increase (P ≤ 0.05) occurring at 10 μg/ml. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|