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Cloning and sequence analysis of Histoplasma capsulatum glucosamine-6-phosphate synthase gene fragment
Authors:Sachadyn  Pawel
Abstract:The 3' part of the glucosamine-6-phosphate synthase gene from Histoplasma capsulatum was PCR amplified using degenerate primers designed from the known glucosamine-6-phosphate synthase gene sequences, cloned and sequenced. The computer analysis of the 676 bp sequence revealed the presence of two introns. The identities of the deduced amino acid sequence to the corresponding Saccharomyces cerevisiae and Candida albicans fragment are 65 and 63.8%, respectively. This revised version was published online in June 2006 with corrections to the Cover Date.
Keywords:l-glutamine  fructose-6-phosphate amidotransferase  Candida albicans  fungi  Saccharomyces cerevisiae  Schizosaccharomyces pombe  systemic mycoses chemotherapy
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