Differential levels of "urotensin-II-like" activity determined by radio-receptor and radioimmuno-assays |
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Authors: | Aiyar Nambi Guida Brian Ao Zhaohui Disa Jyoti Naselsky Diane Behm David J Su Jui-Lan Kull Frederick C Douglas Stephen A |
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Institution: | Department of Vascular Biology and Thrombosis (UW2510), Cardiovascular and Urogenital Center of Excellence for Drug Discovery (CEDD), GlaxoSmithKline, P.O. Box 1539, 709 Swedeland Road, King of Prussia, PA 19406-0939, USA. nambi_aiyar-1@gsk.com |
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Abstract: | Plasma and urinary levels of "urotensin(U)-II-like" substances determined in healthy human volunteers were 12.4 +/- 0.6 ng/ml and 2.2 +/- 0.3 ng/ml by RIA, an order of magnitude lower than that seen by RRA, 167.5 +/- 9.5 ng/ml and 65.2 +/- 4.3 ng/ml. HPLC demonstrated the existence of at least three prominent activity peaks in plasma and urine, the more hydrophobic of which did not co-elute with U-II, degradation products or URP. RRA and RIA recognized these peaks with contrasting efficacy. As such, published levels of "U-II-like" activity should be interpreted with caution until a better understanding is obtained regarding what species specific RIA and RRA assay reagents interact with. |
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