A root-specific O-methyltransferase gene expressed in salt-tolerant barley |
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Authors: | Sugimoto Manabu Okada Yoshihiro Sato Kazuhiro Ito Kazutoshi Takeda Kazuyoshi |
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Affiliation: | Laboratory of Biochemistry, Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan. manabus@rib.okayama-u.ac.jp |
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Abstract: | A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves. |
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