Structural chromosomal rearrangements in HpaII-treated human lymphocytes. |
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Authors: | B Tedeschi B Porfirio D Caporossi P Vernole B Nicoletti |
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Affiliation: | Department of Public Health and Cell Biology, II University of Rome Tor Vergata, Italy. |
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Abstract: | Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrangements are located. |
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