Suitability of AFLP markers for the study of genomic relationships within theOxalis tuberosa alliance |
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Authors: | D S Tosto H E Hopp |
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Institution: | (1) Unidad Integrada Facultad de Ciencias Exactas y Naturales, UBA y Centro Nacional de Investigaciones Agropecuarias (CNIA), INTA, Argentina;(2) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina;(3) Instituto de Biotecnología, CICVyA INTA Castelar, CC 25, 1712 Castelar, Argentina |
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Abstract: | O. tuberosa is an Andean crop that belongs to the worldwide distributed genusOxalis. On the basis of their chromosome numbers the following species (O. herrerae, O. lotoides, O. medicaginea, O. mollissima, O. oblongiformis, O. peduncularis, O. spiralis, O. subintegra, O. tabaconanensis, O. tuberosa, O. villosula) were placed in an alliance. To analyse five species belonging to theOxalis tuberosa alliance (O. oblongiformis, O. peduncularis, O. tabaconanensis, O. tuberosa andO. villosula) and a distant member of the genus (O. articulata), we examined 253 AFLP markers generated after amplification using four primer combinations. Within the alliance, two main clusters were observed, one containing the diploid species and the other group with the polyploid speciesO. tuberosa. All of the primer combinations assayed showed the same clustering pattern. Grouping of accessions of each species by data analysis corresponded largely with their previous taxonomic classifications. The concordance between the clustering of the individuals belonging to different species obtained in this work show the appropriateness of AFLP markers for this type of study. The results obtained are in good agreement with the cytogenetic hypothesis and showed a clustering behaviour, which is similar to the one previously obtained using ITS rDNA nucleotide sequence comparison. |
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Keywords: | Oxalis Oxalis tuberosa oca Andean crop AFLP molecular markers |
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