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聚球藻7942光自养培养的碳代谢和能量代谢
引用本文:颜日明,张志斌,朱笃,储炬.聚球藻7942光自养培养的碳代谢和能量代谢[J].生物工程学报,2009,25(9):1352-1359.
作者姓名:颜日明  张志斌  朱笃  储炬
作者单位:1. 华东理工大学生物反应器国家重点实验室,上海200237;江西师范大学生命科学学院江西省亚热带植物利用与保护重点实验室,南昌330022
2. 江西师范大学生命科学学院江西省亚热带植物利用与保护重点实验室,南昌,330022
3. 江西师范大学生命科学学院江西省亚热带植物利用与保护重点实验室,南昌330022;宜春学院,宜春,336000
4. 华东理工大学生物反应器国家重点实验室,上海,200237
基金项目:国家自然基金项目(No.20506009);;江西省自然科学基金(No.0530095);;江西省教育厅(No.GJJ08147)资助~~
摘    要:代谢通量分析是研究微藻光自养培养过程中CO2和光能利用的一个非常有用的工具。本研究建立了聚球藻7942光自养培养代谢网络,并通过代谢通量方法分析了不同入射光强下的碳代谢流分布和能量代谢。研究结果表明,CO2固定是代谢能量和还原力消耗的主要途径,分别约占总消耗能量的85%和70%。研究还发现在一定光强范围,基于ATP生成的细胞得率和最大细胞得率基本维持不变,分别约为2.80g/molATP和2.95g/molATP,但基于总吸收光能的细胞得率和对应的光能转换效率则随着光强的增加而降低。

关 键 词:聚球藻7942  连续培养  代谢通量分析  能量代谢  光能转化  
收稿时间:2009/5/11 0:00:00

Carbon and energetic metabolism of Synechococcus sp. PCC7942 under photoautotrophic conditions
Riming Yan,Zhibin Zhang,Du Zhu and Ju Chu.Carbon and energetic metabolism of Synechococcus sp. PCC7942 under photoautotrophic conditions[J].Chinese Journal of Biotechnology,2009,25(9):1352-1359.
Authors:Riming Yan  Zhibin Zhang  Du Zhu and Ju Chu
Institution:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 3;Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China;Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China; Yichun University, Yichun 336000, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Abstract:Metabolic flux analysis is a very powerful tool to understand CO2 fixation and light energy utilization of microalgae during photoautotrophic cultivation.A comprehensive network structure for the autotrophic growth of Synechococcus sp.PCC7942 was proposed,and the carbon and energetic metabolism under different incident light intensity was investigated based on metabolic flux analysis in this paper.These results showed that CO2 fixation was the main energy and reducing potential trap which accounted for 85% ...
Keywords:Synechococcus sp  PCC7942  continuous cultivation  metabolic flux analysis  energetic metabolism  light energy conversion  
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