The role of chitinase from Lecanicillium antillanum B-3 in parasitism to root-knot nematode Meloidogyne incognita eggs |
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Authors: | Nam Van Nguyen Young-Ju Kim Kyung-Taek Oh Woo-Jin Jung |
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Institution: | Glucosamine Saccharide Materials – National Research Laboratory, Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology , Chonnam National University , Gwangju , South Korea |
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Abstract: | B-3 fungal isolate was isolated from soil samples of Gwangju in Korea. Based on morphological and phylogenetic analysis, it was designated as Lecanicillium antillanum B-3 (syn. Verticillium antillanum B-3). The fungus was a chitinolytic-nematophagous microorganism. B-3 chitinase activity from 0.5% swollen chitin broth medium reached the highest level on the sixth day and then plateaued until 12 days. B-3 isolate showed the high rate of parasitism on Meloidogyne incognita eggs with more than 90% infection rate on the third day after treatment. B-3 crude chitinase damaged the eggshell structures more than 78% based on lactoglycerol staining data at a final protein concentration of 14.6 µg mL?1 on the fourth day following treatment. Partially purified chitinase with molecular 37 kDa from DEAE-Sephadex chromatography also showed damaging effect on the eggs. These results suggested that chitinase from B-3 isolate was responsible for degradation of M. incognita eggshell structures. |
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Keywords: | Chitinase chitinolytic fungi Lecanicillium antillanum B-3 Meloidogyne incognita |
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