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DNA-identification of rice varieties (<Emphasis Type="Italic">Oriza sativa</Emphasis> L.) of Ukrainian breeding
Authors:M D Bezugliy  Yu M Sivolap  A V Galaev  V V Dudchenko  R A Vozhegova
Institution:1.National Academy of Agrarian Sciences of Ukraine,Kiev,Ukraine;2.South Plant Biotechnology Center,Ukrainian Academy of Agrarian Sciences,Odessa,Ukraine;3.Rice Research Institute,Ukrainian Academy of Agrarian Sciences,Khersons’ka oblast,Ukraine
Abstract:Genetic diversity assessment in 11 rice varieties of the Rice Research Institute’ (Ukrainian Academy of Agrarian Sciences) and also differentiation and identification of 11 rice varieties were conducted by the analysis of 25 SSR-markers. Based on the allele composition of 12 polymorphic microsatellite loci, genetic formulas of examined varieties were developed. UPGMA cluster analysis, with the use of the genetic distances method, allowed for the calculation of differentiation of rice varieties and showed that the Ukrainian rice varieties are genetically similar. As a whole, the use of the SSR-markers was revealed as an effective tool for the assessment of genetic diversity and genotyping within rice varieties of Ukrainian breeding.
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