Oxylipin-coated hat-shaped ascospores of Ascoidea corymbosa |
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Authors: | Ncango Desmond M Pohl Carolina H Sebolai Olihile M Botes Piet J Strauss Catharina J Joseph Manjusha Van Wyk Pieter W J Nigam Santosh Kock Johan L F |
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Affiliation: | UNESCO MIRCEN: Industrial Biotchnology, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa. |
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Abstract: | We previously implicated 3-hydroxy oxylipins and ascospore structure in ascospore release from enclosed asci. Using confocal laser scanning microscopy on cells stained with fluorescein-coupled, 3-hydroxy oxylipin-specific antibodies, we found that oxylipins are specifically associated with ascospores and not the vegetative cells or ascus wall of Ascoidea corymbosa. Using gas chromatography--mass spectrometry the oxylipin 3-hydroxy 17:0 could be identified. Here, we visualize for the first time the forced release of oxylipin-coated, hat-shaped ascospores from terminally torn asci, probably through turgor pressure. We suggest that oxylipin-coated, razor-sharp, hat-shaped ascospore brims may play a role in rupturing the ascus to affect release. |
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