DNA complexes with polycations useful for delivery of DNA into cells |
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| Authors: | N A Kas’yanenko N B Zakharova D A Mukhin A V Slita O V Nazarova E A Leont’eva E F Panarin |
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| Institution: | (1) Institute of Physics, St. Petersburg State University, St. Petersburg, 198504, Russia;(2) Institute of Influenza, Russian Academy of Medical Sciences, St. Petersburg, 197376, Russia;(3) Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, 198004, Russia |
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| Abstract: | Generation and physicochemical properties of complexes formed by high-molecular thymus DNA and plasmid DNA with synthetic polymers of (dimethyl amino)ethyl methacrylate, (diethyl amino)ethyl methacrylate, and poly(vinyl amine) were studied in solutions of different ionic strength using low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The complexes were tested for toxicity with T98G cell cultures. Condensation of DNA was shown to occur when the ratio of charged groups in the polycations and DNA exceeded unity. This condensation manifested itself as an increase in the optical density of DNA solutions. Condensation-associated changes in the dimensions of DNA molecules were determined, and phase diagrams of DNA-polycation systems were analyzed in the presence of NaCl. MTT analysis revealed no toxicity of these complexes. |
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| Keywords: | DNA DNA complexes with synthetic polymers DNA condensation phase diagrams |
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