Induction of yeast apoptosis by an antimicrobial peptide, Papiliocin |
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Authors: | Hwang Bomi Hwang Jae-Sam Lee Juneyoung Kim Jin-Kyoung Kim Seong Ryul Kim Yangmee Lee Dong Gun |
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Institution: | aSchool of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea;bNational Academy of Agricultural Science, RDA, Suwon 441-100, Republic of Korea;cDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea |
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Abstract: | Papiliocin is a 37-residue peptide isolated from the swallowtail butterfly Papilio xuthus. In this study, we found that Papiliocin induced the accumulation of reactive oxygen species (ROS) and hydroxyl radicals known to be important regulators of apoptosis in Candida albicans. To examine the relationship between the accumulation of ROS and the induction of apoptosis, we investigated the apoptotic effects of Papiliocin using apoptotic markers. Cells treated with Papiliocin showed a series of cellular changes normally seen in cells undergoing apoptosis: plasma membrane translocation of phosphatidylserine from the inner to the outer membrane leaflet, measured by Annexin V staining, dissipation of the mitochondrial membrane potential, observed by DiOC6(3) staining; and the presence of active metacaspases, measured using the CaspACE FITC-VAD-FMK, as early apoptotic events. In addition, DNA condensation and fragmentation, which is important marker of late stage apoptosis, was seen by DAPI and TUNEL assay. Therefore, these results suggest that Papiliocin leads to apoptosis in C. albicans via ROS accumulation. |
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Keywords: | Abbreviations: DHR dihydrorhodamine HPF hydroxyphenyl fluorescein FITC fluorescein isothiocyanate PI propidium iodide DiOC6(3) 3 3&prime -dihexyloxacarbocyanine iodide DAPI 4&prime -6-diamidino-2-phenylindole TUNEL terminal deoxynucleotidyltransferase dUTP nick end labeling |
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