CXC chemokine receptor 1 enhances the ability of human umbilical cord blood-derived mesenchymal stem cells to migrate toward gliomas |
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| Authors: | Kim Seong Muk Kim Dal-Soo Jeong Chang Hyun Kim Dong Hyun Kim Ji Hyun Jeon Hong Bae Kwon Soon-Jae Jeun Sin-Soo Yang Yoon Sun Oh Wonil Chang Jong Wook |
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| Affiliation: | aBiomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, South Korea;bDepartment of Neurosurgery, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, South Korea |
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| Abstract: | In this study, we showed that knocking-down interleukin-8 (IL-8) in glioma cells, or its receptor, CXC chemokine receptor 1 (CXCR1) in hUCB-MSCs reduced hUCB-MSC migration toward glioma cells in a Transwell chamber. In contrast, CXCR1-transfected hUCB-MSCs (CXCR1-MSCs) showed a superior capacity to migrate toward glioma cells in a Transwell chamber compared to primary hUCB-MSCs. Furthermore, these transfected cells also demonstrated the same ability to migrate toward tumors in mice bearing intracranial human gliomas as shown by histological and in vivo imaging analysis. Our findings indicate that overexpression of CXCR1 could be a useful tool for MSC-based gene therapy to achieve a sufficient quantity of therapeutic MSCs that are localized within tumors. |
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| Keywords: | Abbreviations: hUCB-MSCS, human umbilical cord blood-derived mesenchymal stem cells IL-8, interleukin-8 CXCR1, CXC chemokine receptor 1 fLuc, firefly luciferase |
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