Inhibition by purine nucleotides of the release of reactive oxygen species from muscle mitochondria: indication for a function of uncoupling proteins as superoxide anion transporters |
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Authors: | Wojtczak Lech Lebiedzińska Magdalena Suski Jan M Więckowski Mariusz R Schönfeld Peter |
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Institution: | aNencki Institute of Experimental Biology, Warsaw, Poland;bInstitut für Biochemie und Zellbiologie, Otto-von-Guericke-Universität Magdeburg, Germany |
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Abstract: | Release of reactive oxygen species (ROS), measured as the sum of hydrogen peroxide (H2O2) and superoxide anion radical ( ), from respiring rat heart and skeletal muscle mitochondria was significantly decreased by millimolar concentrations of GTP or GDP. Attempts to differentiate between the two forms of ROS showed that the release of rather than that of H2O2 was affected. Meanwhile, intramitochondrial ROS accumulation, measured by inactivation of aconitase, increased. These results suggest that guanine nucleotides inhibit the release of from mitochondria. As these nucleotides are known inhibitors of uncoupling proteins (UCPs), it is proposed that UCPs may function as carriers of , thus enabling its removal from the matrix compartment. |
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Keywords: | Abbreviations: CDNB chlordinitrobenzene FCCP carbonylcyanide-p-trifluoromethoxyphenylhydrazone ROS reactive oxygen species SOD superoxide dismutase UCP uncoupling protein |
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