Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines |
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| Authors: | O Felthaus T Ettl M Gosau O Driemel G Brockhoff A Reck K Zeitler M Hautmann TE Reichert G Schmalz C Morsczeck |
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| Institution: | aDepartment of Operative Dentistry and Periodontology, University of Regensburg, Germany;bDepartment of Oral and Maxillofacial Surgery, University of Regensburg, Germany;cDepartment of Gynecology and Obstetrics, University of Regensburg, Germany;dInstitute of Pathology, University of Regensburg, Germany;eDepartment of Radiotherapy, University of Regensburg, Germany |
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| Abstract: | Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133+ cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions. |
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| Keywords: | Cancer stem cells Oral squamous cell carcinoma WNT signaling Colony forming Cell line |
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