Leukocyte-associated immunoglobulin-like receptor-1 is expressed on human megakaryocytes and negatively regulates the maturation of primary megakaryocytic progenitors and cell line |
| |
Authors: | Xue Jiangnan Zhang Xiaoshu Zhao Haiya Fu Qiang Cao Yanning Wang Yuesi Feng Xiaoying Fu Aili |
| |
Institution: | Department of Immunology, Binzhou Medical University, Yantai 264003, China |
| |
Abstract: | Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is an inhibitory collagen receptor which belongs to the immunoglobulin (Ig) superfamily. Although the inhibitory function of LAIR-1 has been extensively described in multiple leukocytes, its role in megakaryocyte (MK) has not been explored so far. Here, we show that LAIR-1 is expressed on human bone marrow CD34+CD41a+ and CD41a+CD42b+ cells. LAIR-1 is also detectable in a fraction of human cord blood CD34+ cell-derived MK that has morphological characteristics of immature MK. In megakaryoblastic cell line Dami, the membrane protein expression of LAIR-1 is up-regulated significantly when cells are treated with phorbol ester phorbol 12-myristate 13-acetate (PMA). Furthermore, cross-linking of LAIR-1 in Dami cells with its natural ligand or anti-LAIR-1 antibody leads to the inhibition of cell proliferation and PMA-promoted differentiation when examined by the MK lineage-specific markers (CD41a and CD42b) and polyploidization. In addition, we also observed that cross-linking of LAIR-1 results in decreased MK generation from primary human CD34+ cells cultured in a cytokines cocktail that contains TPO. These results suggest that LAIR-1 is a likely candidate for an early marker of MK differentiation, and provide initial evidence indicating that LAIR-1 serves as a negative regulator of megakaryocytopoiesis. |
| |
Keywords: | Abbreviations: LAIR-1 leukocyte-associated immunoglobulin-like receptor-1 PMA phorbol ester phorbol 12-myristate 13-acetate ITIM immunoreceptor tyrosine-based inhibition motif |
本文献已被 ScienceDirect PubMed 等数据库收录! |