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Intermedilysin induces EGR-1 expression through calcineurin/NFAT pathway in human cholangiocellular carcinoma cells |
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| Authors: | Susilowati Heni Okamura Hirohiko Hirota Katsuhiko Shono Masayuki Yoshida Kaya Murakami Keiji Tabata Atsushi Nagamune Hideaki Haneji Tatsuji Miyake Yoichiro |
| Institution: | aDepartment of Molecular Pathobiochemistry, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan;bDepartment of Immunopathology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan;cDepartment of Physiology and Biophysics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan |
| Abstract: | RNF8 is a nuclear protein having an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-finger (RF) domain. Depletion of RNF8 caused cell growth inhibition and cell cycle arrest at not only S but also G2/M phases. In addition, cell death was frequently observed in RNF8-depleted cells. Analyses of time-lapse microscopy revealed that the cells died in mitosis and interphase. To elucidate the RNF8 function in M phase, the Plk1 content in RNF8-depleted cells was examined. The amount of RNF8 decreased time-dependently, whereas Plk1 reciprocally increased by transfection of RNF8 siRNA. Protein contents of RNF8 and Plk1 among various cell lines were also compared. RNF8 in normal cell lines was much higher than that in many cancer cell lines. Conversely, Plk1 in normal cell lines was lower than in cancer cell lines. These results suggest that RNF8 is downregulated in many cancer cells and inversely correlated with Plk1. |
| Keywords: | Abbreviations: FHA forkhead-associated RF RING-finger IR ionizing radiation FCS fetal calf serum DAPI 4&prime 6-diamidino-2-phenylindole FITC fluorescein isothiocyanate PBS phosphate buffered saline BSA bovine serum albumin |
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