Membrane penetrating peptides greatly enhance baculovirus transduction efficiency into mammalian cells |
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Authors: | Chen Hong-Zhang Wu Carol P Chao Yu-Chan Liu Catherine Yen-Yen |
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Affiliation: | aInstitute of Biotechnology, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan, ROC;bInstitute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan, ROC |
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Abstract: | The baculovirus group of insect viruses is widely used for foreign gene introduction into mammalian cells for gene expression and protein production; however, the efficiency of baculovirus entry into mammalian cells is in general still low. In this study, two recombinant baculoviruses were engineered and their ability to improve viral entry was examined: (1) cytoplasmic transduction peptide (CTP) was fused with baculovirus envelope protein, GP64, to produce a cytoplasmic membrane penetrating baculovirus (vE-CTP); and (2) the protein transduction domain (PTD) of HIV TAT protein was fused with the baculovirus capsid protein VP39 to form a nuclear membrane penetrating baculovirus (vE-PTD). Transduction experiments showed that both viruses had better transduction efficiency than vE, a control virus that only expresses EGFP in mammalian cells. Interestingly, vE-CTP and vE-PTD were also able to improve the transduction efficiency of a co-transduced baculovirus, resulting in higher levels of gene expression. Our results have described new routes to further enhance the development of baculovirus as a tool for gene delivery into mammalian cells. |
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Keywords: | Baculovirus Transduction Cytoplasmic transduction peptide Protein transduction domain Mammalian cells |
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