Fyn mediates transforming growth factor-beta1-induced down-regulation of E-cadherin in human A549 lung cancer cells |
| |
Authors: | Kim An Na Jeon Woo-Kwang Lim Kyu-Hyoung Lee Hui-Young Kim Woo Jin Kim Byung-Chul |
| |
Affiliation: | aDepartment of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea;bDepartment of Internal Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea |
| |
Abstract: | Transforming growth factor-beta (TGF-β) signaling positively contributes to the regulation of tumor metastasis. However, the underlying molecular mechanisms are less well defined. We here show that Fyn, a member of Src family tyrosine kinases, plays a critical role in mediating TGF-β1-induced down-regulation of E-cadherin in human A549 lung cancer cells. Blockade of Fyn with siRNA knockdown or ligand-binding defective mutant significantly lowered the ability of TGF-β1 to repress E-cadherin expression. Furthermore, our results demonstrated that Fyn facilitates TGF-β1-mediated suppression of E-cadherin through p38 kinase-dependent induction of Snail. Collectively, our findings identify a Fyn-p38-Snail cascade as a new signaling pathway mediating oncogenic TGF-β function. |
| |
Keywords: | Fyn TGF-beta1 E-cadherin Snail p38 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|