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Production of optically pure L-alanine in batch and continuous stirred tank reactors using immobilized Pseudomonas sp.
Authors:H. Hernández  M.L. Asanza  I. Alcaraz  A.M. Hidalgo  M.F. Máximo  J. Bastida
Affiliation:(1) Department of Chemical Engineering, Biochemical Engineering Group, Faculty of Chemistry, University of Murcia, Campus of Espinardo, 30071 Murcia, Spain
Abstract:A process to obtain optically pure l-alanine has been developed using batch and continuous stirred tank reactors with a new l-aminoacylase-producing bacterium Pseudomonas sp. BA2 immobilized in calcium alginate beads coated with glutaraldehyde. The maximum production of l-alanine in a continuous stirred tank reactor was 11.26 g after 2 days of operation which is higher than that previously reported.
Keywords:batch reactor  continuous stirred tank reactor  immobilized cells  l-alaline  l-aminoacylase
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