Adventitious shoot regeneration fromFagus sylvatica leaf explantsin vitro |
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Authors: | Vieitez A M San-José M C |
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Institution: | (1) Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Apartado 122, 15080 Santiago de Compostela, Spain |
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Abstract: | Summary Adventitious shoots were induced on transversally divided expanding leaves fromFagus sylvatica shoot cultures of juvenile origin. Adventitious shoot buds formed mainly on callus that developed on the petiole stump or
on the cut across the midrib of distal leaf halves. However, sometimes they arose directly from leaf tissue. An anatomical
study confirmed both the direct and indirect origin of the adventitious buds. The best results were obtained by culturing
proximal leaf sections on woody plant medium supplemented with 2.9 μM indole-3 acetic acid in combination with 8.9 μM benzyladenine or 2.3 μM thidiazuron (TDZ). Proximal explants were more responsive than distal explants in terms of both callus formation and bud
regeneration, regardless of the induction medium or clone tested. Bud formation capacity was influenced by the genotype of
the stock shoot culture and was enhanced by an initial 10 d darkness, but was inhibited by longer periods of darkness. Caulogenic
competence was significantly affected by the duration of exposure to TDZ; in particular, adventitious shoot length was depressed
by increasing the exposure period. Three weeks culture with TDZ was the most efficient treatment for shoot production and
elongation. Further shoot development was promoted by subculturing the explants to the same medium used for the maintenance
of the stock shoot cultures. Shoots so obtained were multiplied and rooted producing plantlets of adventitious origin. |
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Keywords: | adventitious buds European beech leaf culture shoot regeneration thidiazuron |
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