Identification of a sheath-associated protein involved in phosphate transport in Sphaerotilus natans |
| |
Authors: | Bryan Kaphammer Charles F Kulpa Jr |
| |
Institution: | (1) Department of Biological Sciences, University of Notre Dame, 46556 Notre Dame, Indiana, USA;(2) Present address: 1301 Catherine Medical Science II 5605, University of Michigan, 48109 Ann Arbor, MI, USA |
| |
Abstract: | Summary
Sphaerotilus natans was shown to have a fourfold lower K
mof phosphate transport when grown in medium containing 0.1 mm phosphate, compared to cells grown in 10.0 mm phosphate. Analysis of sheath proteins from cells grown at these two phosphate levels revealed a protein of 53 kDa present in the sheath of cells grown at a phosphate concentration of 0.1 mm. This sheath-associated, phosphate-regulated protein, designated SapP, was gel purified and used to raise a polyclonal antibody. Enzyme-linked immunosorbent assay was used to localize this protein to the surface of the sheathed cells. Phosphate uptake assays done in the presence of the antibody also showed a rise in the K
mof phosphate transport in cells grown in 0.1 mm phosphate, indicating that this protein is involved in high-affinity phosphate transport.Offprint requests to: C. F. Kulpa Jr |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|