Reactivity of basic amino acid pairs in prohormone processing: model of pro-ocytocin/neurophysin processing domain |
| |
Authors: | Lazar Noureddine Brakch Noureddine Panchal Maï Fahy Christine Rholam Mohamed |
| |
Institution: | Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, FRE 2621 CNRS, Université Paris6--Pierre et Marie Curie, 96 Boulevard Raspail, 75006 Paris, France. |
| |
Abstract: | Statistical analysis of several potential dibasic cleavage sites reveals differences in the distribution of basic doublets when the in vivo cleaved sites were compared to those which are not cleaved. Analysis of the substrate specificity of protease Kex2 towards the pro-ocytocin/neurophysin processing domain (pro-OT/Np(7-15) with altered basic pairs shows a cleavage efficiency order in accord with the statistical data. Structural analysis of these substrates indicates that each basic pair is associated with a local and specific conformational change. Thus, the in vivo cleavage hierarchy of dibasic sites is encoded by both the nature of basic pairs and the plasticity of proteolytic processing domains. |
| |
Keywords: | Basic amino acid pairs Kex2 protease Pro-ocytocin/neurophysin processing domain Proteolytic cleavage kinetics Circular dichroism |
本文献已被 ScienceDirect PubMed 等数据库收录! |