Flow cytometric quantification of chlamydial infection in cell culture |
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| Authors: | Michael Grü n, Marita Liebisch, Helga Sauerwein, Gerhard Jahreis,Konrad Sachse |
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| Affiliation: | aInstitute of Nutrition, Friedrich-Schiller-University of Jena, Germany;bFood GmbH, Jena, Germany;cInstitute of Clinical and Molecular Virology, University Medical Center, Erlangen, Germany;dInstitute of Animal Science, University Bonn, Germany;eFriedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Jena, Germany |
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| Abstract: | A flow cytometric method was developed, which allows fast and efficient analysis of cell cultures infected with chlamydiae. The proportion of positive cells increased with the infectious dose and correlated with chlamydia copy numbers calculated from real-time PCR. While retaining the advantages of single-cell analysis, flow cytometry allows handling of large sample numbers and counterstaining for additional marker proteins. |
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| Keywords: | Chlamydia FACS |
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