Cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narB) from Rhodococcus sp. NCIMB12038 |
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Authors: | Kulakov L A Allen C C Lipscomb D A Larkin M J |
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Affiliation: | The Questor Centre, David Keir Building, The Queen's University of Belfast, Stranmillis Road, Belfast, UK. l.kulakov@queens-belfast.ac.uk |
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Abstract: | Rhodococcus sp. NCIMB112038 can utilize naphthalene as its sole carbon and energy source. The gene encoding cis-naphthalene dihydrodiol dehydrogenase (narB) of this strain has been cloned and sequenced. Expression of NCIMB12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in Escherichia coli cells. narB encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from Pseudomonas putida G7. Comparison of NarB with some putative cis-dihydrodiol dehydrogenases from Rhodococcus species revealed significant differences between these proteins. NarB together with two other proteins forms a new group of cis-dihydrodiol dehydrogenases. |
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Keywords: | Pig Diarrhea Pulsed field gel electrophoresis Escherichia coli |
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