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Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2 |
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| Authors: | S Person S C Warner D J Bzik C Debroy B A Fox |
| Institution: | Department of Molecular and Cell Biology 618 Mueller Laboratory The Pennsylvania State University, University Park PA 16802 U.S.A. Tel. (814)865-0382 |
| Abstract: | |
| Keywords: | Recombinant DNA cloned gene inclusion bodies immunological activity subunit vaccine M 13 vector plasmids Ap ampicillin bp base pair(s) cyt cytoplasmic fraction EtBr ethidium bromide gB glycoprotein B HSV IgG immuno γ-globulin IPTG kb kilobase(s) or kilobase pairs mem membrane fraction PAGE polyacrylamide gel electrophoresis PBS phosphateuffered saline resistance SDS sodium dodecyl sulfate TCA trichloroacetic acid TE 10 mM Tris · HC1 pH 8 0 1 mM EDTA V-broth10 g tryptone V-plates V-broth containing 15 g/l agar Xgal To whom correspondence and reprint requests should be addressed |
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