Liver chromatin fractions inMus andAkodon |
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Authors: | Angel Catala Lidia Vidal-Rioja Nestor O Bianchi |
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Institution: | (1) Departmento de Citogenética Molecular, IMBICE, C.C. 403, 1900 La Plata, Argentina |
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Abstract: | Summary The liver chromatin fromMus musculus andAkodon molinae was separated in 8 fractions by differential centrifugation. Like fractions from both species showed approximately similar
contents of DNA, equivalent ratios of histone to non-histone proteins, corresponding template activities and equal amounts
of positive C-banded material. On the other hand, heavy chromatin fractions ofMus were highly enriched in satellite DNA whereas no satellite DNA was found inAkodon chromatin.
Heavy chromatin fractions isolated by differential sedimentation have been usually homologued with the constitutive heterochromatin.
The properties of the constitutive chromatin are discussed and the validity of the foregoing concept is challenged. It is
proposed to define the constitutive heterochromatin as those chromatin regions comprising highly repeated DNA sequences clustered
in restricted areas of chromosomes and not transcribed (satellite DNA). |
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Keywords: | |
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