Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens |
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Authors: | Sung Hun Kim Shukho Kim Sung Guen Chun Mi-Sun Park Jeong Hyun Park Bok-Kwon Lee |
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Affiliation: | Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul 122-701, Republic of Korea. |
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Abstract: | We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken. |
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Keywords: | Salmonella Enteritidis pulsed-field gel electrophoresis phage typing resistance |
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