Explant type and natural ventilation systems influence growth and content of carvacrol and thymol of Lippia gracilis Schauer |
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Authors: | Lazzarini Luiz Eduardo Santos Bertolucci Suzan Kelly Vilela de Carvalho Alexandre Alves Santiago Alexsandro Carvalho Pacheco Fernanda Ventorim Yucesan Buhara Pinto José Eduardo Brasil Pereira |
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Affiliation: | 1.Laboratory of Tissue Culture and Medicinal Plants, Department of Agriculture, Federal University of Lavras, University Campus, Box 3037, Lavras, CEP 37200-000, Brazil ;2.Faculty of Natural and Agricultural Sciences, Department of Seed Science and Technology, Abant Izzet Baysal University, 14280, Bolu, Turkey ; |
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Abstract: | The objective of this study was to evaluate the effects of different natural ventilation systems and explant types on the growth and volatile compound content of Lippia gracilis cultured in vitro. The treatments consisted of four membrane systems (without membrane, with one, two, and four porous membranes) and two explant types (nodal segments with and without a pair of leaves). The evaluation of growth, photosynthetic pigments and chemical analysis of the volatile fraction were performed at 35 days of cultivation in half strength MS basal medium. Natural ventilation systems significantly influenced the in vitro growth and volatile fraction of L. gracilis. Explants with a pair of leaves obtained the best experimental responses. The natural ventilation system with four membranes provided the best growth parameters and leaf area response of L. gracilis explants with leaves. The photosynthetic pigments decreased with an increase in the number of porous membranes in the culture flask. Variations in the number, content, and profile of volatile compounds under the influence of natural ventilation systems were observed. Major constituents such as ρ-cymene, γ-terpinene, thymol, carvacrol, and E-caryophyllene, regardless of experimental conditions, were identified. The highest carvacrol and thymol contents were observed in plantlets grown in culture flasks with four porous membranes. To maximize the content of carvacrol and thymol from the in vitro culture of L. gracilis, explants with a pair of leaves and four porous membranes in culture flasks are recommended for use. |
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